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The ability of HIV to persist within latent cellular reservoirs represents a major barrier to cure. The timing of establishment of individual viral reservoirs over the course of an infection may influence their susceptibility to elimination by immune-mediated or therapeutic approaches. However, methods to accurately estimate the age of reservoir sequences remain scarce. We propose a simple method to date suspected reservoir sequences using phylogenetically informed regression.

Our method is as follows: Taking aligned HIV sequences from a single individual sampled over multiple time points, we reconstruct a maximum-likelihood phylogeny. The location of the root in the phylogeny is determined using root-to-tip regression.

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Positive Singles, one of the leading services connecting daters living with venerial disease, has launched a new area of its site specifically catering to HIV-positive gay individuals. Members of the POZ gay community often find it difficult to connect with others online due to stigma associated with the disease.

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Positive Singles claims over 1,, registered members.

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